In contrast to differentiated cells stem cells appear to rel

2018-10-20

In contrast to differentiated cells, stem Cy3-dCTP appear to rely mainly on glycolysis to generate ATP (Rafalski et al., 2012). Studies analyzing energy metabolism of adult stem cells have focused on oxygen consumption (Pattappa et al., 2011) and mitochondrial activity (Zhang et al., 2013). Further

br Materials and methods br Results br

2018-10-20

Materials and methods Results Discussion We have identified and begun to characterize a novel miRNA, miR-410, which on inhibition of BMP signaling by Noggin, is down-regulated in the adult SVZ. Over-expression of miR-410 in SVZ neurospheres inhibited neuronal differentiation and increased t

Importantly STS and STS have been shown to

2018-10-20

Importantly, STS1 and STS2 have been shown to have overlapping functions (Carpino et al., 2002; Carpino et al., 2004). However, although expansion of LSK, increased serial colony growth, and hyperphosphorylation of FLT3 were largely attributable to the loss of STS1, further loss of STS2 strengthened

Next we attempted to correct the disease causing mutation in

2018-10-20

Next we attempted to correct the disease-causing mutation in a fibroblast line isolated from an infant with severe combined immunodeficiency (SCID), caused by mutations in the gene encoding adenosine deaminase (ADA). SCID patients could particularly benefit from a one-step protocol that facilitates

Importantly because none of these markers

2018-10-20

Importantly, because none of these markers are specific for trophoblast or trophoblast populations in general, they must be used in combination. For example, SWAN-71 and HTR8/SVneo, two widely used “trophoblast” cell lines, are hypermethylated at the ELF5 promoter, HTR8/SVneo does not express C19MC

br Results br Discussion In the present

2018-10-20

Results Discussion In the present study we show that LPA1 is expressed by stem and progenitor Calcium Ionophore I (predominantly type 1 and type 2a) within the neurogenic niche of the dentate gyrus of adult mice, and that it outperforms Nestin, the current gold standard, as a marker for prosp

br Results br Discussion CSL serves as the central

2018-10-20

Results Discussion CSL serves as the central node in canonical Notch signaling by transmitting signaling from all Notch receptors upon ligand activation. In this work, we report that genetic ablation of CSL in breast tumor Tubacin leads to enhanced tumor growth after transplantation into mamma

hmg-coa reductase inhibitor MYC is not a pluripotency

2018-10-20

MYC is not a pluripotency factor, but it substantially improves reprogramming dynamics, primarily because MYC accelerates cell proliferation (Sabo et al., 2014; Walz et al., 2014), providing an opportunity for the somatic genome to gradually reshape its epigenetic landscape. MYC is critical at an ea

Given the significance of methylation demethylation during r

2018-10-20

Given the significance of methylation/demethylation during reprogramming, we set out to investigate the function of miR-29a in regulating the iPSC methylome. Although miR-29a was shown to directly regulate both de novo DNA methyltransferases and demethylases, we found that depletion of miR-29a resul

6XHis In addition to configurations for constitutive gene ex

2018-10-20

In addition to configurations for constitutive gene expression, we also tested the behavior of the Tet-on system (http://www.clontech.com) in which sequences encoding either GFP or mCherry expression were placed under the control of a doxycycline-responsive promoter. Although robust doxycycline-indu

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Introduction Frontotemporal dementia (FTD) refers to a group of neurodegenerative diseases caused by focal but progressive neuronal loss, astrogliosis, and spongiosis in the frontal and temporal cortices associated with abnormal intracellular acc

Mitochondria are highly dynamic organelles with complex stru

2018-10-20

Mitochondria are highly dynamic organelles with complex structures involved in multiple processes, including energy metabolism, reactive oxygen species (ROS) generation, mitochondrial dynamics and distribution, and the induction of apoptosis. A number of recent studies have highlighted the key role

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Acknowledgments This work was supported by grants to W.H. from the National Natural Science Foundation of China (81372258, 81572827), the Major State Basic Research Development Program of China (2013CB910802), and the Program for Professor of Spe

Hello world!

2018-07-29