• In summary we developed a

  2018-10-29

  

  In summary, we developed a powerful strategy generating hemangiogenic FLK-1+ progenitors from ESCs. The unlimited access to hemangioblasts, the ultimate hematopoietic and vascular progenitors, warrants further investigations on potential clinical applications requiring hematopoietic, vascular repair

• In this report we used a high throughput PCR

  2018-10-29

  

  In this report, we used a high-throughput PCR-based platform to examine the similarity between sister glycine receptors due to the difficulty to conduct RNA sequencing analyses for each of over 350 single-cell samples. Interestingly, the average correlation coefficient (0.74) observed for WT ESCs i

• Experimental Procedures br Acknowledgments The authors

  2018-10-29

  

  Experimental Procedures Acknowledgments The authors thank Uri Ben-david, Yonatan Stelzer, Daniel Ronen, Tomer Halevy, and Uri Weissbein for critically reading this manuscript and Tamar Golan-Lev for her assistance with the graphical design of the figures. We thank Yonatan Stelzer for his assista

• Sulfated GAG such as heparan sulfate HS

  2018-10-26

  

  Sulfated GAG, such as heparan sulfate (HS) or chondroitin sulfate (CS), are polysaccharidic molecules associated to a core protein to form proteoglycans, which interact with proteins to strengthen the structure and stability of the extracellular matrix (ECM). GAG also have functional roles since the

• In Raya et al found

  2018-10-26

  

  In 2009, Raya et al. found that somatic MLN0128 from FA patients could only be reprogrammed after correction of the genetic defect, suggesting an important role for the FA pathway in establishing pluripotency (Raya et al., 2009). However, Tulpule et al. subsequently created the first human-specific

• Acknowledgments This work was supported by the

  2018-10-26

  

  Acknowledgments This work was supported by the Agency for Science, Technology and Research, Singapore. We would like to thank the Cytogenetics laboratory at Singapore General Hospital for karyotyping the iPSC lines and Luqman Hakim for help in characterizing the iPSC lines. We would like to thank T

• Neurogenesis in the adult mammalian brain occurs

  2018-10-26

  

  Neurogenesis in the adult mammalian new occurs throughout life and has been clearly demonstrated at two locations under physiological conditions: the SVZ of the lateral ventricle and the subgranular zone (SGZ) of the DG in the hippocampus (Alvarez-Buylla and Lim, 2004). Several studies have shown t

• qRT PCR showed that the expression of the PRC

  2018-10-26

  

  qRT-PCR showed that the expression of the PRC2 core components Ezh2, Suz12, and Eed was not reduced upon Dicer deletion (Figure 2A). Similarly immunoblotting showed no reduction of EZH2, SUZ12, or JARID2 proteins or global H3K4me3 or H3K27me3 (Figure 2B). Cellular fractionation experiments confirmed

• The reprogramming of somatic cells was originally demonstrat

  2018-10-26

  

  The reprogramming of somatic protoporphyrin ix was originally demonstrated by producing cloned frogs using somatic cell nuclear transfer (SCNT) into Xenopus oocytes (Gurdon et al., 1958). Reprogramming of mammalian somatic cells has also been achieved using SCNT into oocytes, including those of mic

• EZ Cap Reagent GG (3\' OMe) br Experimental Procedures b

  2018-10-26

  

  Experimental Procedures Author Contributions Acknowledgments We thank Toshio Kitamura for PLAT-A cells, and Gregg Duester and Daniel J. Donoghue for discussions. Yoav Altman, Joseph Russo, Edward Monosov, Frederick Lo, Maria Jose Martinez Lopez, Justin D. Blethrow, and Alexey Eroshkin kindl

• Adult stem cells are defined as undifferentiated multipotent

  2018-10-26

  

  Adult stem cells are defined as undifferentiated, multipotent progenitor cells with the capability to both self-renew and generate differentiated progeny throughout the lifetime of an organism (Alonso and Fuchs, 2003; Blanpain and Fuchs, 2006; Fuchs and Segre, 2000; Lavker and Sun, 1983; Lavker et a

• There are a number of potential challenges

  2018-10-26

  

  There are a number of potential challenges to using iPSC and iPSC-derived perk kinase to model human phenotype and disease. Somatic heterogeneity in iPSC lines that can occur during isolation and culture may interfere with examining genetic variants with subtle effects (Fusaki et al., 2009; Interna

• This research adds to the

  2018-10-26

  

  This research adds to the body of evidence showing the long-term health benefits of years of schooling. A recent review of the evidence on the impact of education on health finds health benefits arise from receiving high quality education (Cohen & Syme, 2013). Relating education and literacy, qualit

• br Methods and procedures br Results

  2018-10-26

  

  Methods and procedures Results In Table 1 we present the distribution of the independent variables. In 2010, 45% of women reported that they don’t watch TV while 19% reported that they watch TV less than weekly or at least weekly and 35% said that they watch TV almost daily. This prevalence o

• The notion that pets can facilitate

  2018-10-26

  

  The notion that pets can facilitate social capital goes beyond the more commonly investigated role of pets as a social icebreaker between strangers, or as a catalyst for social interaction (Hunt, Hart, & Gomulkiewicz, 1992; McNicholas & Collis, 2000; Messent, 1983; Newby, 1997; Robins, Sanders, & Ca

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