hmg-coa reductase inhibitor MYC is not a pluripotency

2018-10-20

MYC is not a pluripotency factor, but it substantially improves reprogramming dynamics, primarily because MYC accelerates cell proliferation (Sabo et al., 2014; Walz et al., 2014), providing an opportunity for the somatic genome to gradually reshape its epigenetic landscape. MYC is critical at an ea

Given the significance of methylation demethylation during r

2018-10-20

Given the significance of methylation/demethylation during reprogramming, we set out to investigate the function of miR-29a in regulating the iPSC methylome. Although miR-29a was shown to directly regulate both de novo DNA methyltransferases and demethylases, we found that depletion of miR-29a resul

6XHis In addition to configurations for constitutive gene ex

2018-10-20

In addition to configurations for constitutive gene expression, we also tested the behavior of the Tet-on system (http://www.clontech.com) in which sequences encoding either GFP or mCherry expression were placed under the control of a doxycycline-responsive promoter. Although robust doxycycline-indu

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Introduction Frontotemporal dementia (FTD) refers to a group of neurodegenerative diseases caused by focal but progressive neuronal loss, astrogliosis, and spongiosis in the frontal and temporal cortices associated with abnormal intracellular acc

Mitochondria are highly dynamic organelles with complex stru

2018-10-20

Mitochondria are highly dynamic organelles with complex structures involved in multiple processes, including energy metabolism, reactive oxygen species (ROS) generation, mitochondrial dynamics and distribution, and the induction of apoptosis. A number of recent studies have highlighted the key role

br Experimental Procedures br Author

2018-10-20

Experimental Procedures Author Contributions Acknowledgments This work was supported by grants to W.H. from the National Natural Science Foundation of China (81372258, 81572827), the Major State Basic Research Development Program of China (2013CB910802), and the Program for Professor of Spe

Hello world!

2018-07-29